Peptide microarrays coupled to machine learning reveal individual epitopes from human antibody responses with neutralizing capabilities against SARS-CoV-2기계 학습과 결합된 펩티드 마이크로어레이는 SARS-CoV-2에 대한 중화 기능을 가진 인간 항체 반응의 개별 에피토프를 나타냅니다.Article Published on 2022-12-012022-09-11 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 진단, 치료기술, 치료법, [키워드] anti-SARS-CoV-2 antibody Antibody binding assays binding epitope capture causative agent cellular coronavirus coronavirus SARS-CoV-2 Course COVID-19 detectable epitope Epitopes human antibody response IgA IgG IgM antibody immunoassays incubated infected with SARS-CoV-2 machine learning Microarray N-terminus negative control samples neutralization neutralization assays Neutralizing Neutralizing antibodies nine overlapping Patient peptide peptide arrays peptides positive protective effect Protective effects random Random forest response S protein SARS CoV SARS CoV-2 SARS-CoV-2 sera serology Serology. Specific structural protein structural proteins the disease the S protein vaccine candidate vaccine candidates Viral [DOI] 10.1080/22221751.2022.2057874 PMC 바로가기 [Article Type] Article
Biophysical Modeling of SARS-CoV-2 Assembly: Genome Condensation and BuddingArticle Published on 2022-09-202022-11-16 Journal: Viruses [Category] SARS, 유전자 메커니즘, [키워드] assembly budding caused cellular condensation coronavirus 2 COVID-19 pandemic curtail de novo develop drug target envelope Genome genome condensation genomic RNA host cell identify Infectious virus Interaction intrinsic M protein mechanism membranes modeling molecular Mutation N protein oligomerization pathogen Protein protein intrinsic curvature. Research respiratory responsible resulting RNA SARS-CoV-2 spike structural protein structural proteins the S protein therapeutic target therapy Vaccines viral entry viral life cycle viral particle virus while [DOI] 10.3390/v14102089 PMC 바로가기
Thiopurines inhibit coronavirus Spike protein processing and incorporation into progeny virionsArticle Published on 2022-09-192022-11-15 Journal: PLoS Pathogens [Category] SARS, 치료제, [키워드] 6-Thioguanine ACE2-expressing cells acting antiviral activity antiviral drug antivirals approach betacoronavirus betacoronaviruses biochemical Cell complementary coronavirus demonstrated early stage ectopic expression Effect full-length Genetic GTPase had no HCoV-OC43 HPRT1 in vitro infect Infection inhibit inhibitor lentivirus nucleotide oligosaccharide outcome progeny virion Protein pseudotyped pseudovirus Replication SARS-CoV-2 selective shown spike structural proteins subgenomic RNA target Treatment Viral diseases viral genomes Virus-like particle VLPs yielding [DOI] 10.1371/journal.ppat.1010832 PMC 바로가기
Utility of in silico-identified-peptides in spike-S1 domain and nucleocapsid of SARS-CoV-2 for antibody detection in COVID-19 patients and antibody productionArticle Published on 2022-09-052022-11-15 Journal: Scientific Reports [Category] SARS, 변종, 진단, 치료기술, [키워드] 2D structure 3D structure analyzed antibody Antibody detection antibody production Antibody Response Antigen antigenic peptides changes in COVID-19 patient COVID-19 patients domain eight Evidence homology immunogenic in silico analysis limitation N protein New nucleocapsid peptide performed Proteins provide RBD Result S1 domain SARS-CoV-2 SARS-CoV-2 variant selected sequence serum serum sample Seven Standardization structural proteins the N protein the spike protein trimer virus viruses were used [DOI] 10.1038/s41598-022-18517-w PMC 바로가기
Clinical features and severe acute respiratory syndrome-coronavirus-2 structural protein-based serology of Mexican children and adolescents with coronavirus disease 20192019년 코로나바이러스 질환이 있는 멕시코 어린이 및 청소년의 임상 특징 및 중증 급성 호흡기 증후군-코로나바이러스-2 구조 단백질 기반 혈청학Article Published on 2022-08-152022-09-12 Journal: PLoS ONE [Category] SARS, 진단, [키워드] acute respiratory syndrome analyzed Antibody Response Asymptomatic cause children clinical clinical feature Comorbidities Comorbidity coronavirus disease COVID-19 disease E protein exhibited feature heterogeneous population highest Immunosuppression Infection leukemia levels of antibodies lowest majority Mild Mild-to-moderate number of symptoms obesity Outpatient peptide prevalence of COVID-19 SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike serological response serology structural proteins the antibody response treated with COVID-19 [DOI] 10.1371/journal.pone.0273097 PMC 바로가기 [Article Type] Article
Roles and functions of SARS-CoV-2 proteins in host immune evasion숙주 면역 회피에서 SARS-CoV-2 단백질의 역할과 기능Review Published on 2022-08-082022-09-11 Journal: Frontiers in Immunology [Category] SARS, 치료법, [키워드] accessory protein accessory proteins activator acute respiratory syndrome acute respiratory syndrome coronavirus coronavirus described ENCODE evade Frame function functions Genome Host host immune system IFN-β IFNs immune immune evasion inhibiting interferon interferons IRF3 mechanism mechanisms NF-κB nine non-structural protein non-structural proteins nsp1 Nsp12 nsp13 nsp14 Nsp15 nsp5 NSP6 NSP8 Nsps nuclear nuclear translocation Open reading frame ORF10 ORF3b ORF6 ORF8 Orf9b Perspective Phosphorylation Protein Regulatory role SARS-CoV-2 SARS-CoV-2 protein SARS-CoV-2 proteins severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Signaling STAT2 STATs structural proteins structural proteins. suppress TBK1 Transcription type I type I and III interferons variety Viral protein Viral proteins [DOI] 10.3389/fimmu.2022.940756 PMC 바로가기 [Article Type] Review
Omicron mutations enhance infectivity and reduce antibody neutralization of SARS-CoV-2 virus-like particlesOmicron 돌연변이는 SARS-CoV-2 바이러스 유사 입자의 감염성을 높이고 항체 중화를 감소시킵니다.Article Published on 2022-08-022022-09-11 Journal: Proceedings of the National Academy of Sciences of [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition Antibody neutralization Antisera assembly B.1 B.1 lineage B.1.1 bebtelovimab Casirivimab Cell cell entry change convalescent sera coronavirus COVID-19 Delta detectable detrimental dose Effect Efficacy Efficiency eight eight subject elicited ENhance examined Imdevimab individual Johnson & Johnson Johnson & Johnson luciferase Moderna monoclonal antibodies monoclonal antibody Mutation N proteins neutralization neutralization activity neutralization titer Neutralizing antibodies neutralizing antibody omicron Omicron variant Patient Pfizer Pfizer/BioNTech Prevent property reduce reduced respiratory resulting robust SARS-CoV-2 SARS-CoV-2 variant sequence sera severe acute respiratory syndrome Coronavirus Spike protein Spread structural protein structural proteins subjects survivor Vaccine Vaccines variant variants Viral viral infectivity Virus-like particle virus-like particles virus-like particles. VLP VLPs [DOI] 10.1073/pnas.2200592119 PMC 바로가기 [Article Type] Article
Plasmonic Approaches for the Detection of SARS-CoV-2 Viral ParticlesSARS-CoV-2 바이러스 입자 검출을 위한 플라즈몬 접근법Review Published on 2022-07-212022-09-11 Journal: Biosensors [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, 치료법, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition antibody treatment applicability approach Care caused contagious coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 detection diagnostic diagnostic testing diagnostics focus genetic material highlight Highlights Infection infectivity testing neutralizing antibody non-infected nucleocapsid outbreak pandemic Particle Patient platform POCT point-of-care point-of-care testing Protein provided respiratory reverse transcription PCR RT-PCR S1 protein SARS-CoV-2 SARS-CoV-2 viral particle SARS-CoV-2 viral particles SARSC-CoV-2 severe acute respiratory syndrome Coronavirus spike glycoprotein spike glycoproteins Spike protein SPR structural proteins surface plasmon surface plasmon resonance surface plasmonic resonance (SPR). the S1 protein therapeutic Treatment Vaccine Viral viral particle Viral particles Viral protein virus while [DOI] 10.3390/bios12070548 PMC 바로가기 [Article Type] Review
Mutational induction in SARS-CoV-2 major lineages by experimental exposure to neutralising sera중화 혈청에 대한 실험적 노출에 의한 SARS-CoV-2 주요 계통의 돌연변이 유도Article Published on 2022-07-212022-09-11 Journal: Scientific Reports [Category] COVID19(2023년), MERS, SARS, 변종, 유전자 메커니즘, 진단, [키워드] acquisition addition antibody characterisation escape mutation Evolution Evolution of SARS-CoV-2 human population immune evasion immune response Interaction Lineage Mutagenesis neutralisation neutralising neutralising antibody Neutralizing antibody response offer Perspective promoted required responsible SARS-CoV-2 sera species structural protein structural proteins target Viral viral adaptation viral variant [DOI] 10.1038/s41598-022-16533-4 PMC 바로가기 [Article Type] Article
SARS-CoV-2 spike and nucleocapsid proteins fail to activate human dendritic cells or γδ T cellsSARS-CoV-2 스파이크 및 뉴클레오캡시드 단백질은 인간 수지상 세포 또는 γδ T 세포를 활성화하지 못합니다Article Published on 2022-07-142022-09-11 Journal: PLoS ONE [Category] COVID19(2023년), SARS, 유전자 메커니즘, 치료법, [키워드] activate activated acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 CD3+ cell co-cultured contribute coronavirus COVID-19 patient COVID-19 patients Cytokines dendritic cell dendritic cells digestion downstream flow cytometry healthy donors immune immune cells Immunity indicated induce interferon interferon-γ interleukin-12 investigated Ligand mechanism mononuclear cell mononuclear cells nucleocapsid nucleocapsid protein Nucleocapsid proteins protease Protein SARS-CoV-2 SARS-CoV-2 spike severe acute respiratory syndrome Coronavirus stimulated stimulatory structural protein structural proteins T cell T cell activation T cells thought Viral RNA virus [DOI] 10.1371/journal.pone.0271463 PMC 바로가기 [Article Type] Article